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- Mystery Inc.
- Batgirl (single appearance)
- Harley Quinn (single appearance)(main story and flashback)
- Poison Ivy (single appearance)(main story and flashback)
- Ghost 1 (single appearance)(disguise)/
- Catwoman (first full appearance)(final appearance)
- Ghost 2 (single appearance)(disguise)/
- Batgirl (redeemed)
- Batman (mentioned)
- Robin (mentioned)
- Ace the Bat-Hound (mentioned)
- Joker (mentioned)
- Museum guards (single appearance)(no lines)(flashback only)
- Gotham City
- Apartment building
- Museum (flashback only)
- Bank (flashback only)
- Harley Quinn and Poison Ivy heard about the gang from the Joker, who has it out for them after they helped Batman and Robin catch him in the The New Scooby-Doo Movies episodes, The Dynamic Scooby-Doo Affair and The Caped Crusader Caper.
- The character designs of the guest stars are based on Batman: The Animated Series. Catwoman had a cameo appearance in Who's Scared?, where she was based on her Golden Age look from the comics.
- None known.
Inconsistencies/continuity errors and/or goofs/oddities
- None known.